| Expression pattern: |
UP |
| Associated gene: |
CDK1, WTAP, m6A |
| Associated microRNA: |
miR-197-3p |
| Biological function: |
facilitates IL-22-stimulated HaCaT cell proliferation, migration, and invasion; circ_0056856 knockdown represses IL-22-induced HaCaT cell proliferation, migration, and invasion |
| Molecular mechanism: |
WTAP-mediated m6A modification improves circ_0056856 stability; circ_0056856 functions as a sponge of miR-197-3p to modulate CDK1 expression through a ceRNA mechanism |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); ceRNA regulation (promotes); m6A modification (other); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; MeRIP / MeRIP-seq; Transfection; MTT; EdU Staining; Wound Healing Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
circ_0056856 could provide novel insights into psoriasis treatment and a novel avenue of therapy for psoriasis management |
| Description: |
circ_0056856 is up-regulated in psoriasis patient skin samples and IL-22-stimulated HaCaT keratinocytes. WTAP-mediated m6A modification enhances circ_0056856 stability, and circ_0056856 promotes IL-22-induced keratinocyte proliferation, migration, and invasion through the miR-197-3p/CDK1 ceRNA axis. |
| Confidence score: |
0.7795 |