| Expression pattern: |
UP |
| Associated gene: |
KPNA4, STAT3, CCDC66 promoter |
| Associated microRNA: |
miR-33a-5p |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, tumor growth and tumorigenesis, and inhibits apoptosis. |
| Molecular mechanism: |
STAT3 activates CCDC66 transcription to promote circCCDC66 expression; circCCDC66 acts through a ceRNA mechanism by binding miR-33a-5p and positively regulating KPNA4 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Transfection; EdU Staining; Colony Formation Assay; TUNEL; Transwell Assay; Nuclear-Cytoplasmic Fractionation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; ChIP / ChIP-seq; Western Blot; In Vivo Animal Model; Bioinformatics Analysis |
| Clinical significance: |
circCCDC66 is suggested as a promising biomarker and potential molecular targeted therapy-related factor in NSCLC treatment. |
| Description: |
circCCDC66 is up-regulated in NSCLC cells and functions as an oncogenic circRNA that promotes proliferation, migration, invasion, tumor growth and tumorigenesis while suppressing apoptosis. Mechanistically, STAT3 activates CCDC66 transcription to increase circCCDC66 expression, and circCCDC66 promotes NSCLC progression through the miR-33a-5p/KPNA4 ceRNA axis. |
| Confidence score: |
0.8005 |