circRNA basic information
circBase ID: hsa_circ_0001543
Name: hsa_circ_NR3C1
Synonym: KU921432.1 / circ_0001543
Host Gene: NR3C1
Genomic location(hg19): chr5:142779220-142780417:-
Genomic location(hg38): chr5:143399655-143400852:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0004986
MONDO name: urinary bladder carcinoma
Disease details: bladder cancer / BCa
Disease DO ID:
 4007
Disease MeSH ID:
 -
Disease NCIt ID:
 C4912
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

bladder cancer tissues; paired adjacent normal bladder tissues; adjacent normal bladder tissues; xenograft tumors
Cell lines:

T24T; EJ; UMUC3; J82; 5637; SV-HUC-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: cyclin D1, NR3C1 mRNA, CDK2, CDK4, CDK6, CCNE1, P21, P27, Ki67
Associated microRNA: miR-27a-3p, miR-23a-3p
Biological function: Overexpression of circNR3C1 inhibits bladder cancer cell proliferation and cell cycle progression, induces G0/G1 cell cycle arrest, suppresses tumor growth in vivo, and does not affect migration, invasion, or apoptosis in the reported assays.
Molecular mechanism: circNR3C1 functions as a cytoplasmic miRNA sponge for miR-27a-3p, blocking miR-27a-3p interaction with the cyclin D1 mRNA 5'UTR, thereby suppressing cyclin D1 protein expression and inhibiting cell cycle progression and proliferation.
Biological pathway or process:

proliferation (inhibits); cell cycle (inhibits); ceRNA regulation (other)
Detected method:
Q
S
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; Northern Blot; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Cell Cycle Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Western Blot; Bioinformatics Analysis
Clinical significance:

circNR3C1 and miR-27a-3p were suggested as potential therapeutic targets for bladder cancer treatment; lower circNR3C1 expression was not correlated with clinical indicators including bladder cancer grade and pathological stage.
Description:

circNR3C1 is a human NR3C1-derived circRNA downregulated in bladder cancer tissues and cell lines. It acts as an anti-oncogenic cytoplasmic miRNA sponge for miR-27a-3p, reducing cyclin D1 protein expression, inducing G0/G1 arrest, and inhibiting bladder cancer cell proliferation and xenograft tumor growth. The study suggests circNR3C1 as a potential therapeutic target rather than a prognostic marker, since its lower expression was not correlated with grade or pathological stage.
Confidence score:

0.866
Other information
Title:

CircNR3C1 inhibits proliferation of bladder cancer cells by sponging miR-27a-3p and downregulating cyclin D1 expression.

Journal: Cancer letters
Published: 2019
PubMed ID: 31255724
Study type:

combined biological and clinical study
Data availability: GenBank: KU921432.1; CircBase (http://www.circbase.org/)
Code availability: -