circRNA basic information
circBase ID: hsa_circ_0014130
Name: hsa_circ_PIP5K1A
Synonym: circ_PIP5K1A
Host Gene: PIP5K1A
Genomic location(hg19): chr1:151206672-151212515:+
Genomic location(hg38): chr1:151234196-151240039:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

tumor tissues
Cell lines:

A549; H460; A549/DDP; H460/DDP
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: ROCK1
Associated microRNA: miR-493-5p
Biological function: promotes cisplatin resistance and malignant progression (proliferation, cell cycle progression, migration/invasion) and inhibits apoptosis in DDP-resistant NSCLC cells
Molecular mechanism: ceRNA mechanism: circ_PIP5K1A sponges miR-493-5p to upregulate ROCK1
Biological pathway or process:

chemoresistance (promotes); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Transfection; MTT; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Clinical Sample Validation
Clinical significance:

circ_PIP5K1A might be applied as a biomarker to improve the DDP therapy for NSCLC patients.
Description:

circ_PIP5K1A is upregulated in cisplatin (DDP)-resistant NSCLC tissues and resistant cell lines. It promotes chemoresistance and malignant behaviors by sponging miR-493-5p, thereby increasing ROCK1 expression, and it modulates DDP response in a cell line-derived xenograft model.
Confidence score:

0.7857
Other information
Title:

Circ_PIP5K1A regulates cisplatin resistance and malignant progression in non-small cell lung cancer cells and xenograft murine model via depending on miR-493-5p/ROCK1 axis.

Journal: Respiratory research
Published: 2021
PubMed ID: 34537072
Study type:

combined biological and clinical study
Data availability: Please contact the correspondence author for the data request.
Code availability: -