| Expression pattern: |
UP |
| Associated gene: |
mTOR, AGO2, CNOT6L |
| Associated microRNA: |
miR-100 |
| Biological function: |
Promotes ESCC cell proliferation, migration and invasion; associated with lymph node metastasis and advanced TNM stage. |
| Molecular mechanism: |
hsa_circ_0006168 acts through a ceRNA mechanism by sponging miR-100 and regulating mTOR expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Bioinformatics Analysis |
| Clinical significance: |
High hsa_circ_0006168 expression was positively connected with lymph node metastasis and TNM stage; hsa_circ_0006168 can serve as a potential diagnostic biomarker, prognostic biomarker, and therapeutic target for ESCC. |
| Description: |
hsa_circ_0006168 is up-regulated in ESCC tissues and cell lines and is associated with lymph node metastasis and advanced TNM stage. Functionally, it promotes ESCC cell proliferation, migration and invasion. Mechanistically, it acts as a ceRNA by sponging miR-100 and regulating mTOR expression, suggesting potential biomarker and therapeutic relevance. |
| Confidence score: |
0.8324 |