| Expression pattern: |
UP |
| Associated gene: |
E2F3 |
| Associated microRNA: |
miR-432-5p |
| Biological function: |
Promotes breast cancer cell proliferation, cell-cycle progression, migration, and tumor growth in vivo; hsa_circ_0008039 depletion suppresses proliferation, arrests cell-cycle progression, reduces migration, and inhibits tumor growth. |
| Molecular mechanism: |
hsa_circ_0008039 acts as a competing endogenous RNA that sponges miR-432-5p, thereby promoting E2F3 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); cell cycle (promotes); migration (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Transwell Assay; Western Blot; In Vivo Animal Model |
| Clinical significance: |
hsa_circ_0008039 might be a potential therapeutic target for breast cancer therapy. |
| Description: |
hsa_circ_0008039 is up-regulated in breast cancer tissues and cell lines and functions as an oncogenic circRNA. It promotes proliferation, cell-cycle progression, migration, and in vivo tumor growth by acting as a ceRNA for miR-432-5p and enhancing E2F3 expression. The study suggests the hsa_circ_0008039/miR-432-5p/E2F3 axis as a potential therapeutic target for breast cancer. |
| Confidence score: |
0.697 |