| Expression pattern: |
UP |
| Associated gene: |
ZEB2 |
| Associated microRNA: |
miR-1238 |
| Biological function: |
Promotes NSCLC cell proliferation, migration, invasion, metastasis and EMT; inhibits apoptosis; promotes tumor growth in vivo. |
| Molecular mechanism: |
Acts as a ceRNA (miRNA sponge) for miR-1238, thereby de-repressing ZEB2 and activating EMT. |
| Biological pathway or process: |
EMT (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; FISH / smFISH; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; CCK8; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); Clinical Sample Validation; Bioinformatics Analysis |
| Clinical significance: |
Circ-10720 expression was correlated with TNM stage, differentiation and tumor size in NSCLC patients. |
| Description: |
circ-10720 is up-regulated in NSCLC tissues/cells and promotes malignant phenotypes (proliferation, migration/invasion and EMT) while reducing apoptosis. Mechanistically, it functions as a ceRNA that sponges miR-1238, thereby increasing ZEB2 expression and enhancing EMT; circ-10720 knockdown suppresses xenograft tumor growth. |
| Confidence score: |
0.8322 |