| Expression pattern: |
UP |
| Associated gene: |
MDM2, CD133, SOX2, CD90, CD44 |
| Associated microRNA: |
miR-1244 |
| Biological function: |
Promotes osteosarcoma cell proliferation in vivo and in vitro and enhances osteosarcoma cell stemness features. |
| Molecular mechanism: |
circSAMD4A functions as a miR-1244 sponge and regulates the downstream miR-1244 target MDM2, forming a circSAMD4A/miR-1244/MDM2 regulatory loop. |
| Biological pathway or process: |
proliferation (promotes); stemness (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; Nuclear-Cytoplasmic Fractionation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circSAMD4A expression is associated with metastatic tissues and decreased recurrence-free survival, suggesting potential prognostic and therapeutic relevance in osteosarcoma. |
| Description: |
circSAMD4A/hsa_circ_0004846 is up-regulated in osteosarcoma tissues and cell lines and is associated with metastasis and poorer recurrence-free survival. Functionally, it promotes osteosarcoma proliferation and stemness in vitro and accelerates xenograft tumor growth in vivo. Mechanistically, circSAMD4A mainly localizes in the cytoplasm and acts as a miR-1244 sponge to regulate MDM2 expression through a circSAMD4A/miR-1244/MDM2 axis. |
| Confidence score: |
0.8562 |