| Expression pattern: |
UP |
| Associated gene: |
AKT1, GSK-3beta, beta-catenin, c-Myc, cyclin D1, Survivin, Bcl-2 |
| Associated microRNA: |
miR-511 |
| Biological function: |
circZFR promotes HCC cell proliferation, migration, invasion and xenograft tumor growth, and inhibits apoptosis. |
| Molecular mechanism: |
circZFR acts as a miR-511 sponge to up-regulate AKT1 and activate AKT1/beta-catenin signaling and proliferation-related proteins. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); Wnt/beta-catenin (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; Colony Formation Assay; Annexin V/PI Flow Cytometry; Wound Healing Assay; Transwell Assay; Western Blot; IHC (Immunohistochemistry); Luciferase Reporter Assay; In Vivo Animal Model |
| Clinical significance: |
Higher circZFR expression was correlated with tumor size and TNM stage; circZFR is suggested as a potential therapeutic target in HCC. |
| Description: |
circZFR is up-regulated in HCC tissues and cell lines and is associated with larger tumor size and advanced TNM stage. It promotes malignant phenotypes by sponging miR-511, thereby relieving repression of AKT1 and activating AKT1/beta-catenin signaling and proliferation-related proteins. Knockdown of circZFR suppresses proliferation, migration, invasion and xenograft growth while inducing apoptosis, supporting circZFR as a potential therapeutic target in HCC. |
| Confidence score: |
0.6917 |