| Expression pattern: |
UP |
| Associated gene: |
KREMEN1, Ago2 |
| Associated microRNA: |
miR-885-5p |
| Biological function: |
circ_0003611 enhances Abeta-induced neuronal cell injury in AD; knockdown of circ_0003611 relieves apoptosis, inflammation, and oxidative stress and improves cell viability in Abeta-treated SK-N-SH cells. |
| Molecular mechanism: |
circ_0003611 acts as a miR-885-5p sponge to positively regulate KREMEN1 expression, thereby aggravating Abeta-induced neuronal injury. |
| Biological pathway or process: |
apoptosis (promotes); inflammation (promotes); ceRNA regulation (other); other pathway/process (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot; ELISA; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis |
| Clinical significance: |
circ_0003611 upregulation can be used for AD diagnosis and circ_0003611 downregulation may be a novel therapeutic strategy for AD patients. |
| Description: |
circ_0003611 is up-regulated in serum from AD patients and in Abeta-treated SK-N-SH cells. It aggravates Abeta-induced neuronal injury by promoting apoptosis, inflammatory cytokine release, and oxidative damage. Mechanistically, circ_0003611 sponges miR-885-5p to increase KREMEN1 expression, forming a circ_0003611/miR-885-5p/KREMEN1 regulatory axis. |
| Confidence score: |
0.7785 |