| Expression pattern: |
UP |
| Associated gene: |
HOXB2, Bcl-W, A1, Bcl-2, Bad, PI3K, Akt, mTOR, caspase-9 |
| Associated microRNA: |
miR-1200 |
| Biological function: |
circ-0001785 promotes osteosarcoma cell proliferation and tumor growth, inhibits apoptosis, and exerts an oncogenic role in osteosarcoma. |
| Molecular mechanism: |
circ-0001785 functions as a ceRNA by sponging miR-1200 to up-regulate HOXB2 expression, and regulates PI3K/Akt/mTOR signaling and Bcl-2 family-related apoptotic pathways. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); PI3K/AKT/mTOR (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
RNase R Treatment; Sanger Sequencing; RT-qPCR; Microarray; Transfection; EdU Staining; Annexin V/PI Flow Cytometry; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
circ-0001785 may be utilized as a novel hallmark for early diagnosis and treatment of osteosarcoma and as a therapeutic target for osteosarcoma. |
| Description: |
circ-0001785 is up-regulated in osteosarcoma cell lines and acts as an oncogenic circRNA. It promotes proliferation and tumor growth while inhibiting apoptosis, at least partly through a ceRNA mechanism in which circ-0001785 sponges miR-1200 to up-regulate HOXB2 and modulates PI3K/Akt/mTOR and Bcl-2 family-related apoptotic signaling. |
| Confidence score: |
0.7795 |