| Expression pattern: |
UP |
| Associated gene: |
IDO1, MMP-8, CD40, ApoA I, RNASE1 |
| Associated microRNA: |
- |
| Biological function: |
Serves as a potential diagnostic biomarker and independent predictor for CAD; overexpression in THP-1-derived macrophages positively regulates proatherosclerotic gene expression and negatively regulates antiatherosclerotic gene expression, indicating a potential proatherosclerotic effect. |
| Molecular mechanism: |
hsa_circ_0004104 overexpression alters mRNA expression profiles in THP-1-derived macrophages, upregulating proatherosclerotic genes IDO1, MMP-8 and CD40 and downregulating antiatherosclerotic genes ApoA I and RNASE1; corresponding protein changes were confirmed for IDO1, CD40, MMP-8 and ApoA I. |
| Biological pathway or process: |
other pathway/process (promotes); inflammation (promotes); immune regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis; Sanger Sequencing; Transfection; Western Blot; ELISA |
| Clinical significance: |
hsa_circ_0004104 was significantly upregulated in CAD patients, could serve as a potential biomarker for CAD, and was an independent predictor of CAD occurrence with AUC 0.700. |
| Description: |
hsa_circ_0004104 is upregulated in PBMCs from CAD patients and was validated as a potential diagnostic biomarker and independent predictor for CAD. Functionally, its overexpression in THP-1-derived macrophages promotes a proatherosclerotic expression program by increasing IDO1, MMP-8 and CD40 and decreasing ApoA I and RNASE1, suggesting contribution to atherosclerosis and CAD pathogenesis. |
| Confidence score: |
0.7242 |