| Expression pattern: |
UP |
| Associated gene: |
interleukin 17 receptor D (IL17RD) messenger RNA, IL17RD, IL17RD 3′-UTR |
| Associated microRNA: |
miR-518a-5p |
| Biological function: |
Facilitates glioma cell growth, migration, and invasion, and inhibits cell apoptosis; acts as an oncogenic circRNA in glioma cells. |
| Molecular mechanism: |
circ-PITX1 sponges miR-518a-5p and releases miR-518a-5p-mediated repression of IL17RD, thereby upregulating IL17RD at the posttranscriptional level. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
High circ-PITX1 expression was correlated with larger tumor size and more advanced World Health Organization grade; circ-PITX1 may be used as a therapeutic target for glioma. |
| Description: |
circ-PITX1 is upregulated in glioma tissues and cell lines, and its high expression is associated with larger tumor size and advanced WHO grade. Functionally, circ-PITX1 promotes glioma cell growth, migration, and invasion while inhibiting apoptosis. Mechanistically, it acts as a ceRNA by sponging miR-518a-5p to relieve repression of IL17RD, forming a circ-PITX1/miR-518a-5p/IL17RD oncogenic axis. |
| Confidence score: |
0.6536 |