| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
miR-136 |
| Biological function: |
circRNA_100876 promotes osteosarcoma cell proliferation and tumor growth; knockdown suppresses proliferation in vitro and in vivo, promotes apoptosis, arrests cells at G2/M stage, and has no significant effect on migration. |
| Molecular mechanism: |
circRNA_100876 directly binds miR-136; silencing circRNA_100876 increases miR-136, and miR-136 inhibitors reverse the anti-proliferation effect caused by circRNA_100876 knockdown. The functional effects involve apoptosis promotion and G2/M cell-cycle arrest after circRNA_100876 downregulation. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Luciferase Reporter Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
circRNA_100876 is upregulated in osteosarcoma tissues, correlates with tumor size, tumor differentiation degree and shorter overall survival time, and may serve as a promising biomarker and treatment target for osteosarcoma. |
| Description: |
circRNA_100876 is upregulated in osteosarcoma tissues and cell lines, and high expression is associated with larger tumor size, poor differentiation, and shorter overall survival. Functionally, circRNA_100876 promotes osteosarcoma cell proliferation and tumor growth, while its knockdown induces apoptosis and G2/M cell-cycle arrest. Mechanistically, circRNA_100876 directly binds miR-136, and inhibition of miR-136 reverses the growth suppression caused by circRNA_100876 silencing. |
| Confidence score: |
0.713 |