circRNA basic information
circBase ID: hsa_circ_0072309
Name: hsa_circ_LIFR
Synonym: -
Host Gene: -
Genomic location(hg19): chr5:38523520-38530768:-
Genomic location(hg38): chr5:38523418-38530666:-
Subcellular localization: nucleus
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tissue samples; paracancerous tissues; plasma samples; serum samples
Cell lines:

THLE-2; Huh-7; Hep 3B2.1-7
In vivo animal model:

-
circRNA-disease information
Expression pattern:
DN
Associated gene: LIFR, p300, H3K27ac
Associated microRNA: -
Biological function: Suppresses HCC cell proliferation, migration, invasion, and sorafenib resistance; promotes sorafenib sensitivity; acts as a tumor suppressor in HCC.
Molecular mechanism: hsa_circ_0072309 forms a positive feedback regulatory loop with its host gene LIFR. It recruits p300 to promote H3K27ac enrichment in the LIFR enhancer, thereby transcriptionally promoting LIFR expression; LIFR overexpression promotes hsa_circ_0072309 formation.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); drug resistance (inhibits); other pathway/process (promotes)
Detected method:
Q
M
Validation methods:

RT-qPCR; Microarray; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; Luciferase Reporter Assay; ChIP / ChIP-seq; RIP (RNA Immunoprecipitation); ROC Analysis; Bioinformatics Analysis
Clinical significance:

Serum hsa_circ_0072309 may serve as a noninvasive diagnostic marker for HCC; low hsa_circ_0072309 expression is associated with larger tumor size, lymph node metastasis/vascular invasion, satellite nodules, and elevated alpha-fetoprotein levels.
Description:

hsa_circ_0072309 is downregulated in HCC tissues, serum samples, and HCC cell lines, and serum expression shows diagnostic potential. Functionally, it suppresses HCC cell proliferation, migration, invasion, and sorafenib resistance. Mechanistically, hsa_circ_0072309 binds p300 and promotes H3K27ac enrichment at the LIFR enhancer to activate LIFR transcription, forming a positive feedback regulatory loop with its host gene LIFR.
Confidence score:

0.8625
Other information
Title:

hsa_circ_0072309 Inhibits Oncogenesis in Hepatocellular Carcinoma by Epigenetic Activation of its Host Gene.

Journal: Cell biochemistry and biophysics
Published: 2024
PubMed ID: 39283585
Study type:

combined biological and clinical study
Data availability: GSE242797; GSE216115; TCGA-LIHC; GEPIA webtool (http://gepia2.cancer-pku.cn/#index); KM-plotter (https:// kmplot.com/analysis/index.php?p=background); https://doi.org/10.1007/s12013-024-01330-9
Code availability: -