circRNA basic information
circBase ID: -
Name: hsa_circ_CCDC66
Synonym: circRNA_CCDC66
Host Gene: CCDC66
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: cytoplasm; extracellular vesicle
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
 5672, 9256
Disease MeSH ID:
 -
Disease NCIt ID:
 C4978
Disease ICD11 ID:
 -
Disease OMIM ID:
 114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

-
Cell lines:

THP-1; HEK-293 T; SW480; HCT116; FHC; CD8 + T cells
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: MTDH mRNA, MTDH, PDL1
Associated microRNA: miR-342-3p
Biological function: M2 macrophage-derived EV-delivered circ_CCDC66 promotes CRC cell proliferation, migration, invasion, tumorigenic activity, and immune evasion; suppression of circ_CCDC66 blocks CRC cell growth and mobility.
Molecular mechanism: M2-EVs deliver circ_CCDC66 into CRC cells, where circ_CCDC66 acts as a ceRNA sponge for miR-342-3p to restore MTDH mRNA expression, contributing to PDL1-associated immune evasion and CRC development.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); immune regulation (promotes); ceRNA regulation (promotes); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; EdU Staining; Transwell Assay; Western Blot; In Vivo Animal Model
Clinical significance:

-
Description:

This study shows that M2 macrophage-derived extracellular vesicles are enriched in circ_CCDC66 and can deliver it into CRC cells. circ_CCDC66 promotes CRC proliferation, migration, invasion, xenograft tumor growth, and immune evasion by sponging miR-342-3p and restoring MTDH expression, which is linked to reduced CD8+ T-cell cytotoxicity and increased PDL1.
Confidence score:

0.657
Other information
Title:

M2 macrophage-derived extracellular vesicles augment immune evasion and development of colorectal cancer via a circRNA_CCDC66/microRNA-342-3p/metadherin axis.

Journal: Cytotechnology
Published: 2023
PubMed ID: 37389129
Study type:

biological research
Data availability: All the data generated or analyzed during this study are included in this published article.
Code availability: -