circRNA basic information
circBase ID: hsa_circ_0000735
Name: hsa_circ_P2RX1
Synonym: circ_0000735
Host Gene: P2RX1
Genomic location(hg19): chr17:3802927-3808661:-
Genomic location(hg38): chr17:3899633-3905367:-
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: Non-small-cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissue; matched nontumorous tissue; tumor tissue
Cell lines:

SK-MES-1; H1299; H1755; A549; H1581; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: BMPER
Associated microRNA: miR-940
Biological function: Promotes proliferation, migration, invasion, glycolysis and tumor growth in NSCLC; circ_0000735 knockdown suppresses these malignant phenotypes.
Molecular mechanism: Acts as a miRNA sponge to modulate miR-940 and thereby regulate BMPER expression (ceRNA mechanism).
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; Survival Analysis; Clinical Sample Validation
Clinical significance:

High circ_0000735 expression is associated with shorter overall survival and may be a prognostic biomarker in NSCLC.
Description:

circ_0000735 is upregulated in NSCLC and functions as an oncogenic circRNA, promoting proliferation, migration, invasion, glycolysis, and xenograft tumor growth. Mechanistically, it primarily localizes to the cytoplasm and regulates BMPER expression by sponging miR-940, forming a circ_0000735/miR-940/BMPER axis associated with poor patient survival.
Confidence score:

0.7855
Other information
Title:

Downregulation of Hsa_circ_0000735 Inhibits the Proliferation, Migration, Invasion, and Glycolysis in Non-small-cell Lung Cancer by Targeting miR-940/BMPER Axis.

Journal: OncoTargets and therapy
Published: 2020
PubMed ID: 32922033
Study type:

combined biological and clinical study
Data availability: -
Code availability: -