| Expression pattern: |
UP |
| Associated gene: |
ANKRD20A9P, KCNQ1OT1, XIST |
| Associated microRNA: |
hsa-miR-561-5p, hsa-miR-140-3p |
| Biological function: |
Associated with declining ovarian reserve and adverse embryo outcomes; shows ROC predictive performance for pregnancy outcomes after ART. |
| Molecular mechanism: |
Predicted miRNA sponge (ceRNA) mechanism based on bioinformatics. |
| Biological pathway or process: |
glycolysis (other); cell cycle (other); ovarian steroidogenesis (other); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Sanger Sequencing; Back-Splice Junction PCR / divergent primers PCR; Clinical Sample Validation; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
circRNA_104816 expression in granulosa cells is negatively correlated with top quality embryos and has ROC predictive performance for clinical pregnancy and live birth in ART cycles. |
| Description: |
circRNA_104816 (hsa_circRNA_104816, from IARS) is up-regulated in granulosa cells from older women and is associated with reduced ovarian reserve measures and poorer embryo outcomes. It has ROC-based predictive value for clinical pregnancy and live birth after ART, and bioinformatics suggests it may act via a ceRNA mechanism involving miRNAs and predicted targets such as ANKRD20A9P, KCNQ1OT1, and XIST, linked to glucose metabolism, mitotic cell cycle, and ovarian steroidogenesis. |
| Confidence score: |
0.6841 |