| Expression pattern: |
DN |
| Associated gene: |
PROX1, NOTCH1, HES1, AGO2 |
| Associated microRNA: |
miR-181b-5p |
| Biological function: |
Promotes human neural cell migration and ENCC migration and proliferation; knockdown impairs ENCC proliferation and migration. |
| Molecular mechanism: |
ceRNA mechanism: circTIMMDC1 sponges miR-181b-5p to regulate PROX1 and the NOTCH1/HES1 signaling pathway. |
| Biological pathway or process: |
ceRNA regulation (promotes); Notch (inhibits); migration (promotes); proliferation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; FISH / smFISH; Clinical Sample Validation; Bioinformatics Analysis; Transfection; Wound Healing Assay; Transwell Assay; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); RNA Pull-Down; Western Blot; EdU Staining; In Vivo Animal Model |
| Clinical significance: |
May act as a novel therapeutic target for HSCR. |
| Description: |
circTIMMDC1 is downregulated in HSCR patient tissues. It promotes neural cell and ENCC migration and proliferation by sponging miR-181b-5p and modulating the PROX1-NOTCH1-HES1 axis, acting synergistically with circANKRD12 and suggesting potential therapeutic relevance in HSCR. |
| Confidence score: |
0.7519 |