circRNA basic information
circBase ID: hsa_circ_0000284
Name: hsa_circ_HIPK3
Synonym: CircRNA HIPK3 / circHIPK3
Host Gene: HIPK3
Genomic location(hg19): chr11:33307958-33309057:+
Genomic location(hg38): chr11:33286412-33287511:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: Hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

plasma
Cell lines:

HepG2; Huh7; LX-2
In vivo animal model:

-
circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: -
Biological function: Promotes tumor metastasis and enhances proliferation and invasion of HCC cells; upregulated in HCC cell lines and patient plasma.
Molecular mechanism: Acts as a sponge for miRNA (ceRNA-like).
Biological pathway or process:

proliferation (promotes); invasion (promotes); metastasis (promotes)
Detected method:
Q
Validation methods:

divergent primers PCR; RNase R Treatment; Sanger Sequencing; ddPCR; RT-qPCR; Clinical Sample Validation; ROC Analysis
Clinical significance:

Higher circHIPK3 level in plasma of HCC patients than healthy individuals; suggested potential biomarker/liquid biopsy target.
Description:

circHIPK3 (hsa_circ_0000284) is up-regulated in HCC patient plasma and in HCC cell lines (HepG2, Huh7) versus normal liver cells (LX-2). This study develops and benchmarks a ddPCR assay (vs RT-qPCR) to sensitively quantify plasma circHIPK3, supporting its use as a liquid-biopsy biomarker candidate for HCC.
Confidence score:

0.6605
Other information
Title:

Development and Evaluation of a Droplet Digital PCR Assay for the Accurately Detecting the CircHIPK3 in Plasma Samples from Patients with Hepatocellular Carcinoma.

Journal: Journal of microbiology and biotechnology
Published: 2025
PubMed ID: 40374541
Study type:

combined biological and clinical study
Data availability: http://jmb.or.kr; Data are available from the authors upon reasonable request and with permission from the Centre for Ethics Committee of Ningbo No.2 Hospital.
Code availability: -