| Expression pattern: |
UP |
| Associated gene: |
Ago2 protein |
| Associated microRNA: |
miR-217, miR-485-3p |
| Biological function: |
Enhances 5-FU resistance in colorectal cancer, promotes proliferation under 5-FU treatment, inhibits apoptosis, and transfers chemoresistance to 5-FU-sensitive CRC cells via exosomes. |
| Molecular mechanism: |
Exosome-mediated transfer of circ_0000338 enhances 5-FU resistance by directly binding and negatively regulating miR-217 and miR-485-3p. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); drug resistance (promotes); chemoresistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Western Blot; In Vivo Animal Model; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Exosomal circ_0000338 is higher in serum of 5-FU-resistant CRC patients and has diagnostic efficiency for 5-FU resistance. |
| Description: |
circ_0000338 is up-regulated in 5-FU-resistant colorectal cancer tissues, cells, and serum exosomes. It promotes 5-FU resistance by suppressing miR-217 and miR-485-3p, increasing proliferation and reducing apoptosis under 5-FU treatment. Exosome-mediated transfer of circ_0000338 spreads chemoresistance to sensitive CRC cells and shows diagnostic potential for identifying 5-FU-resistant CRC patients. |
| Confidence score: |
0.8568 |