circRNA basic information
circBase ID: hsa_circ_0004873
Name: hsa_circ_OXCT1
Synonym: circ-OXCT1
Host Gene: OXCT1
Genomic location(hg19): chr5:41794104-41807540:-
Genomic location(hg38): chr5:41794002-41807438:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; lung adenocarcinoma tissues; lung squamous carcinoma tissues; paired adjacent normal tissues; NSCLC tumor tissues; tumor tissues of nude mice
Cell lines:

A549; H1975; H1650; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: SLC1A5, Ago2
Associated microRNA: miR-516b-5p
Biological function: Promotes NSCLC cell proliferation, migration, invasion, tumor growth and glutamine metabolism, and inhibits apoptosis.
Molecular mechanism: circ-OXCT1 acts as a molecular sponge for miR-516b-5p and upregulates SLC1A5, thereby facilitating NSCLC progression and glutamine metabolism.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); glutaminolysis (promotes); ceRNA regulation (other); other pathway/process (promotes)
Detected method:
Q
Validation methods:

RNase R Treatment; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; IHC (Immunohistochemistry); In Vivo Animal Model; Bioinformatics Analysis
Clinical significance:

provided a possible circRNA-targeted therapy for NSCLC
Description:

circ-OXCT1 is upregulated in NSCLC tissues and cell lines. It promotes malignant phenotypes and glutamine metabolism while suppressing apoptosis, acting through a circ-OXCT1/miR-516b-5p/SLC1A5 regulatory axis. Knockdown of circ-OXCT1 also inhibits xenograft tumor growth, suggesting therapeutic relevance.
Confidence score:

0.8006
Other information
Title:

CircRNA OXCT1 promotes the malignant progression and glutamine metabolism of non-small cell lung cancer by absorbing miR-516b-5p and upregulating SLC1A5.

Journal: Cell cycle (Georgetown, Tex.)
Published: 2023
PubMed ID: 35482822
Study type:

combined biological and clinical study
Data availability: available from the corresponding author on reasonable request
Code availability: -