circRNA basic information
circBase ID: hsa_circ_0074854
Name: hsa_circ_MAT2B
Synonym: circular RNA MAT2B / circMAT2B
Host Gene: MAT2B
Genomic location(hg19): chr5:162940560-162944680:+
Genomic location(hg38): chr5:163513554-163517674:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

HCC tumorous tissue; corresponding adjacent non-tumorous tissue; serum; lung metastatic tissue; xenograft tumor tissue
Cell lines:

HepG2; Huh7; SMMC-7721; Focus; MHCC-97L; MHCC-97H; L02
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PKM2, KIFC1, AGO2
Associated microRNA: miR-338-3p
Biological function: Promotes HCC glycolysis, glucose utilization, tumor growth, proliferation, migration, invasion, metastasis and malignancy under hypoxia; inhibits apoptosis.
Molecular mechanism: circMAT2B acts as a miRNA sponge for miR-338-3p, thereby up-regulating the miR-338-3p target gene PKM2 and promoting glycolysis and HCC progression under hypoxia; it also modulates KIFC1.
Biological pathway or process:

glycolysis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); apoptosis (inhibits); ceRNA regulation (promotes); mitochondrial function (inhibits); other pathway/process (promotes)
Detected method:
Q
H
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High circMAT2B expression predicts poor overall survival and is correlated with tumor size, vascular invasion, tumor multiplicity, tumor encapsulation, tumor-node-metastasis stage, Edmonson stage, and higher PET/CT SUVmax in HCC patients.
Description:

circMAT2B is up-regulated in HCC tissues and cell lines and high expression predicts poor overall survival. It promotes glycolysis, tumor growth, migration, invasion and metastasis under hypoxia by sponging miR-338-3p and up-regulating PKM2, suggesting therapeutic potential in HCC.
Confidence score:

0.8981
Other information
Title:

Circular RNA MAT2B Promotes Glycolysis and Malignancy of Hepatocellular Carcinoma Through the miR-338-3p/PKM2 Axis Under Hypoxic Stress.

Journal: Hepatology (Baltimore, Md.)
Published: 2019
PubMed ID: 31004447
Study type:

combined biological and clinical study
Data availability: GSE97332; GSE94508; GSE78520; TCGA
Code availability: -