circRNA basic information
circBase ID: hsa_circ_0090049
Name: -
Synonym: Circular RNA_0090049
Host Gene: -
Genomic location(hg19): chrX:21869626-21871621:+
Genomic location(hg38): chrX:21851508-21853503:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007256
MONDO name: hepatocellular carcinoma
Disease details: hepatocellular carcinoma / HCC
Disease DO ID:
 684, 686
Disease MeSH ID:
 D006528
Disease NCIt ID:
 C3099
Disease ICD11 ID:
 1294035808
Disease OMIM ID:
 114550
Species: Human
Species details: Homo sapiens
Tissue specimen:

Liver tumor specimens; para-hepatic normal liver tissue; HCC tissues; xenografts
Cell lines:

THLE-2; Huh7; HCCLM3
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: UBE2T
Associated microRNA: miR-605-5p, miR-548c-3p
Biological function: Promotes HCC cell proliferation, migration, invasion, tumor sphere formation, EMT-related malignant behavior, stemness-associated protein expression, and tumor growth in vivo.
Molecular mechanism: circ_0090049 functions as a miRNA sponge for miR-605-5p and miR-548c-3p and positively regulates UBE2T expression, promoting HCC progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); stemness (promotes)
Detected method:
Q
Validation methods:

RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; IHC (Immunohistochemistry)
Clinical significance:

circ_0090049 may be a potential cure target for HCC.
Description:

circ_0090049 is upregulated in HCC tissues and cell lines and mainly localized in the cytoplasm. It promotes malignant HCC phenotypes, including proliferation, migration, invasion, tumor sphere formation, EMT-associated changes, stemness marker expression, and xenograft tumor growth. Mechanistically, circ_0090049 acts as a sponge for miR-605-5p and miR-548c-3p to increase UBE2T expression, forming circ_0090049/miR-605-5p/UBE2T and circ_0090049/miR-548c-3p/UBE2T regulatory axes.
Confidence score:

0.7859
Other information
Title:

The expression of circ_0090049 in hepatocellular carcinoma and the molecular regulation mechanism of other biological functions.

Journal: Anti-cancer drugs
Published: 2022
PubMed ID: 34620742
Study type:

combined biological and clinical study
Data availability: Please contact the correspondence author for the data request.
Code availability: -