| Expression pattern: |
UP |
| Associated gene: |
PODXL |
| Associated microRNA: |
miR-762 |
| Biological function: |
Promotes LAC cell proliferation, migration, invasion and facilitates EMT. |
| Molecular mechanism: |
Acts as a miRNA sponge (ceRNA) for miR-762 to upregulate PODXL, thereby promoting LAC progression and EMT. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Clinical Sample Validation; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Western Blot; IHC (Immunohistochemistry) |
| Clinical significance: |
High has_circ_0002360 expression is associated with tumor differentiation and lymphatic metastasis, and may be a prognostic tool and therapeutic target for LAC. |
| Description: |
has_circ_0002360 is overexpressed in lung adenocarcinoma tissues and cell lines and promotes proliferation, migration, invasion and EMT. It functions as a ceRNA by sponging miR-762, thereby relieving repression of PODXL and driving LAC progression. |
| Confidence score: |
0.8166 |