| Expression pattern: |
UP |
| Associated gene: |
MDM2 |
| Associated microRNA: |
miR-944 |
| Biological function: |
promotes NPC cell proliferation and invasion |
| Molecular mechanism: |
Acts as a ceRNA by sponging miR-944 to regulate MDM2 expression |
| Biological pathway or process: |
proliferation (promotes); invasion (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Survival Analysis; Nuclear-Cytoplasmic Fractionation; Bioinformatics Analysis; RNA Pull-Down; Luciferase Reporter Assay; Transfection; Colony Formation Assay; CCK8; Transwell Assay; IHC (Immunohistochemistry); Western Blot |
| Clinical significance: |
High circSERPINA3 expression was associated with advanced clinical stage, lymph node metastasis, and poor overall survival (OS) in NPC patients. |
| Description: |
circSERPINA3 (hsa_circ_0033074), derived from SERPINA3, is up-regulated in NPC tissues and cell lines and promotes NPC cell proliferation and invasion. Mechanistically, it mainly localizes to the cytoplasm and functions as a ceRNA by sponging miR-944 to de-repress MDM2, forming the circSERPINA3/miR-944/MDM2 axis. High circSERPINA3 expression correlates with advanced stage/lymph-node metastasis and poorer overall survival. |
| Confidence score: |
0.7967 |