circRNA basic information
circBase ID: hsa_circ_0001666
Name: hsa_circ_FAM120B
Synonym: circ0001666
Host Gene: FAM120B
Genomic location(hg19): chr6:170626457-170639638:+
Genomic location(hg38): chr6:170317369-170330550:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; adjacent non-tumor tissues
Cell lines:

BEAS-2B; A549; H1299; H322; HCC2279
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: AGO1, AGO2
Associated microRNA: miR-1184, miR-548I
Biological function: promotes migration and invasion
Molecular mechanism: Acts as a miRNA sponge for miR-1184 and miR-548I to upregulate AGO1 and activate PI3K/AKT/mTOR signaling.
Biological pathway or process:

migration (promotes); invasion (promotes); PI3K/AKT/mTOR (promotes); metastasis (promotes); ceRNA regulation (promotes)
Detected method:
Q
S
Validation methods:

RNA-seq; RT-qPCR; Clinical Sample Validation; Survival Analysis; divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; Transwell Assay; Wound Healing Assay; Western Blot; Bioinformatics Analysis; In Vivo Animal Model; H&E Staining
Clinical significance:

High expression of circ0001666 was positively associated with lymph node metastasis and pathological stage in NSCLC patients; high circ0001666 expression level had a poor survival time.
Description:

hsa_circ_0001666 (circ0001666), derived from FAM120B, is upregulated in NSCLC tissues and cell lines and is mainly localized in the cytoplasm. It promotes NSCLC migration and invasion in vitro and metastasis in a mouse model by sponging miR-1184 and miR-548I, thereby upregulating AGO1 and activating PI3K/AKT/mTOR signaling; high expression is associated with lymph node metastasis, advanced stage, and poorer survival.
Confidence score:

0.8745
Other information
Title:

Hsa_circ_0001666 promotes non-small cell lung cancer migration and invasion through miR-1184/miR-548I/AGO1 axis.

Journal: Molecular therapy oncolytics
Published: 2022
PubMed ID: 35284630
Study type:

combined biological and clinical study
Data availability: GEO: GSE101586; https://doi.org/10.1016/j.omto.2022.02.011
Code availability: -