| Expression pattern: |
UP |
| Associated gene: |
Raf1, MEK1/2, ERK1/2 |
| Associated microRNA: |
miR-7-5p |
| Biological function: |
Promotes DNA synthesis, cell proliferation, invasion, migration, growth and metastasis of breast cancer cells; inhibits apoptosis; downregulation induces G2 phase arrest and cell apoptosis. |
| Molecular mechanism: |
circ_0006528 acts as a competing endogenous RNA and endogenous sponge of miR-7-5p, reducing miR-7-5p activity, promoting Raf1 expression, and activating the MAPK/ERK signalling pathway through the circ_0006528/miR-7-5p/Raf1 axis. |
| Biological pathway or process: |
MAPK (promotes); ERK (promotes); proliferation (promotes); invasion (promotes); migration (promotes); metastasis (promotes); apoptosis (inhibits); cell cycle (promotes); ceRNA regulation (promotes); drug resistance (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Transwell Assay; Wound Healing Assay; Luciferase Reporter Assay; Western Blot; Bioinformatics Analysis; Cohort Study; Survival Analysis |
| Clinical significance: |
circ_0006528 expression was associated with advanced TNM stage and poor prognosis; high circ_0006528 expression was associated with lower relapse-free survival and overall survival rates; circ_0006528 may serve as a biomarker for predicting prognosis and a therapeutic target. |
| Description: |
circ_0006528 is upregulated in human breast cancer tissues and is associated with advanced TNM stage and poor prognosis. Functionally, it promotes breast cancer cell DNA synthesis, proliferation, invasion, migration, growth and metastasis while inhibiting apoptosis. Mechanistically, circ_0006528 acts as a ceRNA by sponging miR-7-5p, thereby promoting Raf1 expression and activating the MAPK/ERK signalling pathway. |
| Confidence score: |
0.7414 |