| Expression pattern: |
UP |
| Associated gene: |
SIRT1, Sirtuin 1, Wnt/beta-catenin signaling pathway |
| Associated microRNA: |
miR-135a-5p |
| Biological function: |
Promotes LAC cell growth, proliferation and tumor growth; inhibits apoptosis; knockdown suppresses LAC cell growth in vitro and in vivo. |
| Molecular mechanism: |
circ_0001946 acts as a cytoplasmic molecular sponge of miR-135a-5p to upregulate SIRT1, thereby activating the SIRT1/Wnt/beta-catenin signaling pathway. |
| Biological pathway or process: |
Wnt/beta-catenin (promotes); proliferation (promotes); apoptosis (inhibits); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; RT-qPCR; Microarray; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; circRIP; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Western Blot; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0001946 expression is correlated with advanced TNM stage, larger tumor size and low overall survival rate; circ_0001946 might be a potential biomarker for diagnosis or treatment of LAC. |
| Description: |
circ_0001946 is upregulated in lung adenocarcinoma tissues and cell lines, and high expression is associated with advanced TNM stage, larger tumor size and poor overall survival. Functionally, circ_0001946 promotes LAC cell proliferation and tumor growth while inhibiting apoptosis. Mechanistically, cytoplasmic circ_0001946 sponges miR-135a-5p to upregulate SIRT1 and activate Wnt/beta-catenin signaling. |
| Confidence score: |
0.866 |