| Expression pattern: |
UP |
| Associated gene: |
INHBA, HuR |
| Associated microRNA: |
miR-204-5p |
| Biological function: |
promotes proliferation, migration, invasion, EMT, tumor growth and lung metastasis in gastric cancer |
| Molecular mechanism: |
circTHBS1 sponges miR-204-5p to upregulate INHBA and also binds HuR to enhance HuR-mediated INHBA mRNA stability and promote HuR cytoplasmic export, activating the TGF-beta pathway |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); TGF-beta/SMAD (promotes); metastasis (promotes); ceRNA regulation (promotes); mRNA stability (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Actinomycin D / DRB Stability Assay; Clinical Sample Validation; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; IF (Immunofluorescence); Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
high circTHBS1 expression is associated with poor prognosis in gastric cancer and may be a diagnostic/prognostic biomarker |
| Description: |
circTHBS1 (hsa_circ_0034536) is up-regulated in gastric cancer and promotes proliferation, EMT, invasion, and metastasis. It increases INHBA expression through dual mechanisms: sponging miR-204-5p (ceRNA) and binding HuR to enhance INHBA mRNA stability, thereby activating TGF-beta/SMAD signaling and associating with poor prognosis. |
| Confidence score: |
0.8933 |