circRNA basic information
circBase ID: -
Name: hsa_circ_PACRGL
Synonym: circPACRGL
Host Gene: PACRGL
Genomic location(hg19): -
Genomic location(hg38): -
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0005575
MONDO name: colorectal cancer
Disease details: colorectal cancer / CRC
Disease DO ID:
5672, 9256
Disease MeSH ID:
-
Disease NCIt ID:
C4978
Disease ICD11 ID:
-
Disease OMIM ID:
114500
Species: Human
Species details: Homo sapiens
Tissue specimen:

CRC tissues; paired normal tissues; tumor tissues; adjacent normal tissues

Cell lines:

SW480; SW620; LOVO; HT29; NCM460

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: CNBP
Associated microRNA: miR-330-3p
Biological function: circPACRGL promotes CRC cell proliferation, migration, invasion and tumor growth, and inhibits apoptosis.
Molecular mechanism: circPACRGL functions as a ceRNA/miRNA sponge for miR-330-3p to upregulate CNBP expression, thereby promoting CRC progression.
Biological pathway or process:

ceRNA regulation (promotes); proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits)

Detected method:
Q
Validation methods:

RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Clinical Sample Validation; RIP (RNA Immunoprecipitation); Luciferase Reporter Assay; Transfection; MTT; EdU Staining; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Wound Healing Assay; IHC (Immunohistochemistry); Western Blot; In Vivo Animal Model; Bioinformatics Analysis

Clinical significance:

circPACRGL is highlighted as a potential therapeutic target for colorectal cancer.

Description:

circPACRGL is up-regulated in colorectal cancer tissues and cells. It promotes CRC proliferation, migration, invasion and in vivo tumor growth while suppressing apoptosis, mainly by sponging miR-330-3p to increase CNBP expression. The study suggests circPACRGL may serve as a potential therapeutic target for colorectal cancer.

Confidence score:

0.8299

Other information
Title:

CircPACRGL promoted cell proliferation, migration and invasion as well as inhibited cell apoptosis in colorectal cancer via regulation of the miR-330-3p/CNBP axis.

Journal: Molecular and cellular biochemistry
Published: 2023
PubMed ID: 36459268
Study type:

combined biological and clinical study

Data availability: -
Code availability: -