circRNA basic information
circBase ID: hsa_circ_0001142
Name: hsa_circ_PIGU
Synonym: circ_0001142 / circ-PIGU
Host Gene: PIGU
Genomic location(hg19): chr20:33148403-33245340:-
Genomic location(hg38): chr20:34560599-34657536:-
Subcellular localization: cytoplasm; exosome
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0007254
MONDO name: breast cancer
Disease details: breast cancer / BC
Disease DO ID:
 1612
Disease MeSH ID:
 -
Disease NCIt ID:
 C9335
Disease ICD11 ID:
 1047754165
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

breast cancer tissues; adjacent normal breast tissues; tumor tissues; mouse tumor tissues; liver; lungs
Cell lines:

MCF10A; MDA-MB-231; MB231; MDA-MB-453; MB453; BT-20; BT-549; THP-1
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PIK3CB, PI3K/AKT pathway, exosomes, macrophages, autophagy
Associated microRNA: miRNA-361-3p, miR-361-3p
Biological function: Promotes breast cancer cell proliferation, migration, invasion, glycolysis, tumor growth and metastasis; inhibits apoptosis; induces M2 polarization of macrophages and regulates autophagy in tumor-associated macrophages.
Molecular mechanism: circ_0001142 acts as an exosomal circRNA and ceRNA sponge for miR-361-3p, upregulates PIK3CB, activates the PI3K/AKT pathway, inhibits autophagy, and induces M2 macrophage polarization; endoplasmic reticulum stress promotes tumor exosome secretion and circ_0001142 transfer.
Biological pathway or process:

proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); EMT (promotes); glycolysis (promotes); apoptosis (inhibits); autophagy (inhibits); PI3K/AKT (promotes); immune regulation (promotes); macrophage polarization (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; RIP (RNA Immunoprecipitation); RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Cell Cycle Assay; Flow Cytometry（Non-apoptosis/cycle）; Transwell Assay; TUNEL; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); IF (Immunofluorescence); ELISA; Western Blot; Bioinformatics Analysis
Clinical significance:

Overexpression of circ_0001142 was associated with advanced TNM stage, lymphatic metastasis and poor prognosis; circ_0001142 may be a potential biomarker for diagnosis, prognosis and therapy of breast cancer.
Description:

circ_0001142/hsa_circ_0001142 is up-regulated in breast cancer tissues and cell lines and functions as an oncogenic circRNA. It is transferred from breast cancer cells to macrophages via exosomes, sponges miR-361-3p to regulate PIK3CB and PI3K/AKT signaling, inhibits autophagy, induces M2 macrophage polarization, and promotes tumor growth, metastasis, EMT and glycolysis. Its high expression is associated with advanced TNM stage, lymphatic metastasis and poor prognosis, suggesting biomarker and therapeutic potential.
Confidence score:

0.8748
Other information
Title:

Endoplasmic reticulum stress promotes breast cancer cells to release exosomes circ_0001142 and induces M2 polarization of macrophages to regulate tumor progression.

Journal: Pharmacological research
Published: 2022
PubMed ID: 35091089
Study type:

combined biological and clinical study
Data availability: GSE182471; GSE110339; Supplementary table S1; Supplementary data associated with this article can be found in the online version at doi:10.1016/j.phrs.2022.106098
Code availability: -