| Expression pattern: |
UP |
| Associated gene: |
PTPN13, ErbB, MAPK |
| Associated microRNA: |
miR-1257, miR-1272, miR-1305, miR-922, miR-4322, miR-4632-5p, miR-6879-5p |
| Biological function: |
biomarker for diagnosis and prognosis; associated with advanced stage and poor differentiation |
| Molecular mechanism: |
predicted ceRNA (miRNA sponge) mechanism based on cytoplasmic localization and bioinformatics prediction; not experimentally confirmed |
| Biological pathway or process: |
MAPK (other); ERK (other); ceRNA regulation (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Actinomycin D / DRB Stability Assay; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; ROC Analysis; Bioinformatics Analysis |
| Clinical significance: |
Serum hsa_circ_0070354 shows diagnostic value for NSCLC (AUC 0.660) and high expression is an independent predictor of poor prognosis (HR = 5.087, p < 0.001). |
| Description: |
hsa_circ_0070354 is up-regulated in NSCLC tissues, cell lines, and patient serum, and mainly localizes to the cytoplasm. It serves as a serum biomarker with moderate diagnostic performance (AUC 0.660) and high expression predicts poor overall survival, acting as an independent prognostic factor; its ceRNA network and MAPK/ErbB-related pathways were bioinformatically predicted but not experimentally confirmed. |
| Confidence score: |
0.7378 |