| Expression pattern: |
DN |
| Associated gene: |
XIAP |
| Associated microRNA: |
miR-146a-5p |
| Biological function: |
Exosomal circZNF292 ameliorates intermittent hypoxia-mediated cardiomyocyte damage, inhibits AC16 cardiomyocyte apoptosis, and its knockdown aggravates IH-induced injury and apoptosis. |
| Molecular mechanism: |
Exosomal circZNF292 negatively regulates miR-146a-5p and acts through the miR-146a-5p/XIAP pathway; bioinformatics prediction and dual-luciferase reporter assay confirmed direct interaction between circZNF292 and miR-146a-5p. |
| Biological pathway or process: |
apoptosis (inhibits); ceRNA regulation (other); other pathway/process (other) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Bioinformatics Analysis; Luciferase Reporter Assay; Transfection; CCK8; Annexin V/PI Flow Cytometry; Western Blot |
| Clinical significance: |
Exosomal circZNF292 may serve as a biomarker and therapeutic target for patients with OSA and cardiovascular disease. |
| Description: |
In an in vitro model of intermittent hypoxia-induced AC16 cardiomyocyte injury, exosomal circZNF292 was down-regulated. The study supports a protective role for exosomal circZNF292, because its knockdown increased miR-146a-5p, reduced the anti-apoptotic context linked to XIAP, and aggravated cardiomyocyte apoptosis. The authors propose the exosomal circZNF292/miR-146a-5p/XIAP pathway as a potential biomarker and therapeutic target in OSA-associated cardiovascular disease. |
| Confidence score: |
0.5554 |