| Expression pattern: |
UP |
| Associated gene: |
METTL3, IGF2BP1, DDX27 mRNA |
| Associated microRNA: |
- |
| Biological function: |
promotes CRC stemness, migration, invasion, EMT, tumor growth and liver metastasis |
| Molecular mechanism: |
METTL3-mediated m6A modification increases circUHRF2; circUHRF2 binds IGF2BP1 and promotes IGF2BP1-DDX27 mRNA interaction, increasing DDX27 mRNA stability and preventing loss of DDX27 protein |
| Biological pathway or process: |
stemness (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); mRNA stability (promotes) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; Nuclear-Cytoplasmic Fractionation; FISH / smFISH; RNA Pull-Down; RIP (RNA Immunoprecipitation); Transfection; Actinomycin D / DRB Stability Assay; Western Blot; Wound Healing Assay; Transwell Assay; Flow Cytometry（Non-apoptosis/cycle）; Colony Formation Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; MeRIP / MeRIP-seq; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
aberrantly increased expression of circUHRF2 was positively correlated with poor prognosis of CRC patients |
| Description: |
circUHRF2 is upregulated in colorectal cancer and is mainly localized in the cytoplasm. It promotes CRC stemness and metastasis by binding IGF2BP1 to enhance DDX27 mRNA stability and maintain DDX27 protein, and its expression is increased by METTL3-mediated m6A modification and correlates with poor survival. |
| Confidence score: |
0.8912 |