| Expression pattern: |
UP |
| Associated gene: |
IGF1R, LARP1B, Bcl-2, MMP9 |
| Associated microRNA: |
miR-578 |
| Biological function: |
circ-LARP1B promotes HCC tumorigenesis, cell proliferation, invasion, angiogenesis and radioresistance, while inhibiting apoptosis and radiosensitivity; circ-LARP1B knockdown suppresses malignant behaviors and enhances radiosensitivity in vitro and in vivo. |
| Molecular mechanism: |
circ-LARP1B acts as a cytoplasmic miRNA sponge/ceRNA that binds miR-578 and relieves miR-578-mediated repression of IGF1R, thereby up-regulating IGF1R and regulating HCC tumorigenicity and radiosensitivity. |
| Biological pathway or process: |
proliferation (promotes); apoptosis (inhibits); invasion (promotes); angiogenesis (promotes); radioresistance (promotes); ceRNA regulation (other) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Annexin V/PI Flow Cytometry; Transwell Assay; Tube Formation Assay; Western Blot; Luciferase Reporter Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ-LARP1B expression is associated with shorter overall survival and poor outcomes in HCC patients, suggesting prognostic value and a potential therapeutic target. |
| Description: |
circ-LARP1B is up-regulated in HCC tissues and cells, and its high expression predicts poor overall survival. Functionally, circ-LARP1B promotes HCC tumorigenic phenotypes and radioresistance; knockdown suppresses proliferation, invasion, angiogenesis and tumor growth while enhancing radiosensitivity. Mechanistically, cytoplasmic circ-LARP1B sponges miR-578 to up-regulate IGF1R. |
| Confidence score: |
0.8156 |