| Expression pattern: |
UP |
| Associated gene: |
TPT1, Mcl-1, Bcl-xL, Bax |
| Associated microRNA: |
miR-145-5p |
| Biological function: |
Promotes NFPA cell proliferation, colony formation, migration, invasion and xenograft tumor growth; inhibits apoptosis through sponging miR-145-5p and upregulating TPT1 signaling. |
| Molecular mechanism: |
circOMA1 acts as a competing endogenous RNA sponge for miR-145-5p, reducing miR-145-5p activity and thereby upregulating TPT1 and downstream Mcl-1/Bcl-xL/Bax signaling. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); apoptosis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RT-qPCR; FISH / smFISH; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; Western Blot; IHC (Immunohistochemistry); Bioinformatics Analysis |
| Clinical significance: |
circOMA1 expression is higher in invasive NFPA tissues than in noninvasive tissues and positively correlates with tumor invasiveness; the circOMA1-miR-145-5p-TPT1 axis might serve as a potential therapeutic target. |
| Description: |
circOMA1 is upregulated in NFPAs, especially invasive tumors. It promotes NFPA cell proliferation, invasion and xenograft tumor growth by sponging miR-145-5p, thereby increasing TPT1 and downstream anti-apoptotic signaling. |
| Confidence score: |
0.8174 |