| Expression pattern: |
DS |
| Associated gene: |
SNF8, ENO1, ENO2 |
| Associated microRNA: |
- |
| Biological function: |
Tumor-suppressive; inhibits proliferation, migration, invasion, EMT and metastasis; regulates intracellular ROS and metabolic pathways. |
| Molecular mechanism: |
circRHOBTB3 binds metabolic enzymes (ENO1/ENO2) to regulate metabolism and ROS, thereby inhibiting EMT; SNF8 (ESCRT-II) binds circRHOBTB3 and sorts it into exosomes via specific motifs (141-240 nt) enabling exosomal secretion; ASOs targeting circularization/secretion elements increase intracellular circRHOBTB3 and suppress CRC phenotypes. |
| Biological pathway or process: |
metastasis (inhibits); EMT (inhibits); proliferation (inhibits); oxidative phosphorylation (other); oxidative phosphorylation (other); NF-kappaB (other); ERK (other); other pathway/process (other) |
| Detected method: |
Q
S
|
| Validation methods: |
RNA-seq; RT-qPCR; divergent primers PCR; Back-Splice Junction PCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Survival Analysis; Bioinformatics Analysis; Transfection; RNA Pull-Down; Western Blot; RIP (RNA Immunoprecipitation); CCK8; Transwell Assay; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Flow Cytometry（Non-apoptosis/cycle） |
| Clinical significance: |
circRHOBTB3 is higher in CRC patient serum than healthy donors, downregulated in CRC tissues, and higher tissue expression is associated with better overall survival. |
| Description: |
circRHOBTB3 (hsa_circ_0007444) is down-regulated in CRC tumor tissues but elevated in patient serum exosomes. Functionally, circRHOBTB3 suppresses CRC proliferation, migration/invasion, EMT and metastasis by regulating metabolism and ROS, partly through binding ENO1/ENO2, and it is sorted into exosomes via SNF8-dependent motifs; blocking its exosomal export and/or boosting its circularization with ASOs enhances antitumor effects. |
| Confidence score: |
0.8814 |