| Expression pattern: |
UP |
| Associated gene: |
- |
| Associated microRNA: |
hsa-miR-28-5p, ebv-miR-BART20-5p, ebv-miR-BART13-5p |
| Biological function: |
ebv-circRPMS1 likely serves as a miRNA sponge, downregulates human miRNA expression, promotes cell migration, and may regulate host and/or viral gene expression. |
| Molecular mechanism: |
Potential miRNA sponge mechanism; possible nuclear gene transcription regulation. |
| Biological pathway or process: |
ceRNA regulation (promotes); migration (promotes); other pathway/process (other) |
| Detected method: |
Q
H
S
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; RNA-seq; ISH (In Situ Hybridization); Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Bioinformatics Analysis; Transfection; Wound Healing Assay; In Vivo Animal Model |
| Clinical significance: |
ebv-circRPMS1 was detected in human EBVaGC tissue and NPC tissue, suggesting potential relevance to EBV-associated malignancies. |
| Description: |
This study identified ebv-circRPMS1 as an EBV-encoded circular RNA derived from exons 2-4 of the RPMS1 gene and detected it in EBV-positive cell lines, xenografts, EBVaGC tissue, and NPC tissue. ebv-circRPMS1 localizes in both the cytoplasm and nucleus, may function as a miRNA sponge for human and EBV miRNAs, downregulates several human miRNAs upon overexpression, and promotes AGS cell migration in wound healing assays. |
| Confidence score: |
0.7562 |