| Expression pattern: |
UP |
| Associated gene: |
BMI-1 |
| Associated microRNA: |
miR-330-3p |
| Biological function: |
promotes proliferation, migration, invasion, EMT, tumor growth and metastasis in prostate cancer |
| Molecular mechanism: |
Acts as a competing endogenous RNA (miRNA sponge) for miR-330-3p, thereby relieving repression of BMI-1 and increasing BMI-1 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); EMT (promotes); metastasis (promotes); ceRNA regulation (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; Bioinformatics Analysis; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Luciferase Reporter Assay; Transfection; Western Blot; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Clinical Sample Validation; Survival Analysis |
| Clinical significance: |
High circ-0016068 expression is associated with lower survival rates and may be a diagnostic biomarker for early stage prostate cancer. |
| Description: |
circ-0016068 is up-regulated in prostate cancer tissues and cell lines and functions as an oncogenic circRNA. It mainly localizes to the cytoplasm and promotes proliferation, migration, invasion, EMT, and xenograft tumor growth/metastasis by sponging miR-330-3p to de-repress BMI-1. |
| Confidence score: |
0.8427 |