| Expression pattern: |
UP |
| Associated gene: |
HNRNPC, GPX4 |
| Associated microRNA: |
- |
| Biological function: |
promotes RCC cell proliferation, migration/invasion and metastasis; induces ferroptosis |
| Molecular mechanism: |
circASAP1 binds HNRNPC in the cytoplasm and regulates HNRNPC protein stability via the ubiquitin-proteasome pathway, leading to reduced GPX4 mRNA stability/protein level and ferroptosis pathway activation. |
| Biological pathway or process: |
ferroptosis (promotes); proliferation (promotes); migration (promotes); invasion (promotes); metastasis (promotes); mRNA stability (other) |
| Detected method: |
Q
S
|
| Validation methods: |
circRNA-seq; Bioinformatics Analysis; RT-qPCR; RNase R Treatment; Actinomycin D / DRB Stability Assay; FISH / smFISH; Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNA Pull-Down; RIP (RNA Immunoprecipitation); Western Blot; Transfection; CCK8; EdU Staining; Colony Formation Assay; Transwell Assay; Wound Healing Assay; Flow Cytometry（Non-apoptosis/cycle）; In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry); Survival Analysis; Clinical Sample Validation; Cohort Study |
| Clinical significance: |
High expression of circASAP1 was associated with poor prognosis and metastasis in ccRCC; high circASAP1 expression had worse overall and disease-free survival. |
| Description: |
circASAP1 is up-regulated in ccRCC and promotes malignant phenotypes (proliferation, migration/invasion, metastasis). Mechanistically, it binds cytoplasmic HNRNPC and modulates its ubiquitin-proteasome-related protein stability, thereby reducing GPX4 mRNA stability/protein levels and promoting ferroptosis; clinically, high circASAP1 associates with metastasis and poor survival. |
| Confidence score: |
0.8833 |