| Expression pattern: |
UP |
| Associated gene: |
ALCAM |
| Associated microRNA: |
miR-148a-3p |
| Biological function: |
Promotes the migration and invasion abilities of human adipose-derived MSCs after treatment with AGS cell-derived exosomes. |
| Molecular mechanism: |
circ_0004303 acts as a ceRNA/miR-148a-3p sponge to upregulate ALCAM expression, thereby enhancing migration and invasion of human adipose-derived MSCs. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); ceRNA regulation (promotes); metastasis (promotes) |
| Detected method: |
Q
M
|
| Validation methods: |
Microarray; RT-qPCR; Sanger Sequencing; RNase R Treatment; Nuclear-Cytoplasmic Fractionation; Luciferase Reporter Assay; Transfection; Transwell Assay; Western Blot; Bioinformatics Analysis |
| Clinical significance: |
- |
| Description: |
In this study, AGS gastric cancer cell-derived exosomes induced upregulation of hsa_circ_0004303 in human adipose-derived MSCs. Elevated circ_0004303 promoted MSC migration and invasion by sponging miR-148a-3p and increasing ALCAM expression, suggesting a mechanism by which gastric cancer cells may enhance MSC homing and tumor-promoting phenotypes. |
| Confidence score: |
0.7211 |