circRNA basic information
circBase ID: hsa_circ_0016760
Name: -
Synonym: circ_0016760
Host Gene: -
Genomic location(hg19): chr1:227935392-227947186:+
Genomic location(hg38): chr1:227747691-227759485:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: Non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

human fresh NSCLC tissues; normal adjacent tissues; subcutaneous tumors
Cell lines:

A549; H358; H1299; H1975; 16HBE
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: GAGE1
Associated microRNA: miR-1287
Biological function: Promotes NSCLC cell growth, proliferation, colony formation, migration, invasion and tumor growth in vivo, and inhibits apoptosis.
Molecular mechanism: circ_0016760 acts as a ceRNA by sponging miR-1287, thereby upregulating GAGE1 expression and promoting NSCLC progression.
Biological pathway or process:

proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Luciferase Reporter Assay; Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

Upregulation of circ_0016760 is associated with advanced TNM stages, lymph node metastasis and adverse prognosis; high expression predicts shorter overall survival and circ_0016760 may function as an independent prognostic factor for NSCLC patients after surgery.
Description:

circ_0016760 is upregulated in NSCLC tissues and cell lines, and its high expression is associated with advanced TNM stage, lymph node metastasis, and poor overall survival. Functionally, circ_0016760 promotes proliferation, colony formation, migration, invasion, and xenograft tumor growth while inhibiting apoptosis. Mechanistically, it acts as a ceRNA for miR-1287 to increase GAGE1 expression, forming a circ_0016760/miR-1287/GAGE1 regulatory axis that contributes to NSCLC progression.
Confidence score:

0.7412
Other information
Title:

Upregulated circular RNA circ_0016760 indicates unfavorable prognosis in NSCLC and promotes cell progression through miR-1287/GAGE1 axis.

Journal: Biochemical and biophysical research communications
Published: 2018
PubMed ID: 30103946
Study type:

combined biological and clinical study
Data availability: -
Code availability: -