circRNA basic information
circBase ID: hsa_circ_0009910
Name: hsa_circ_MFN2
Synonym: circ_0009910 / hsa_circRNA_100053
Host Gene: -
Genomic location(hg19): chr1:12049221-12052747:+
Genomic location(hg38): chr1:11989164-11992690:+
Subcellular localization: cytoplasm
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
0018874
MONDO name: acute myeloid leukemia
Disease details: acute myeloid leukemia / AML
Disease DO ID:
9119
Disease MeSH ID:
D015470
Disease NCIt ID:
C3171
Disease ICD11 ID:
-
Disease OMIM ID:
601626
Species: Human
Species details: Homo sapiens
Tissue specimen:

bone marrow samples; bone marrow aspiration

Cell lines:

1D3; Mono-Mac-6; KG-1; AML2; AML5

In vivo animal model:

cell line-derived xenograft

circRNA-disease information
Expression pattern:
UP
Associated gene: -
Associated microRNA: miR-20a-5p
Biological function: circ_0009910 promotes AML cell growth and proliferation, supports G1-S cell cycle progression, and inhibits apoptosis; knockdown of circ_0009910 inhibits AML cell proliferation and tumor growth and induces apoptosis.
Molecular mechanism: circ_0009910 functions as a sponge for miR-20a-5p; circ_0009910 knockdown increases miR-20a-5p expression, thereby suppressing AML cell growth.
Biological pathway or process:

ceRNA regulation (other); proliferation (promotes); apoptosis (inhibits); cell cycle (promotes)

Detected method:
Q
M
Validation methods:

Back-Splice Junction PCR / divergent primers PCR; Microarray; RT-qPCR; FISH / smFISH; Clinical Sample Validation; RNA Pull-Down; Luciferase Reporter Assay; Transfection; CCK8; Cell Cycle Assay; Annexin V/PI Flow Cytometry; In Vivo Animal Model; Cohort Study; Survival Analysis; Bioinformatics Analysis

Clinical significance:

High circ_0009910 expression predicted poor risk and worse outcome/prognosis in AML patients; circ_0009910 might serve as a novel outcome biomarker for AML.

Description:

circ_0009910 is up-regulated in AML bone marrow samples and leukemia cell lines, and high expression is associated with poor-risk disease and shorter overall survival. Functionally, circ_0009910 promotes AML5 cell proliferation, cell cycle progression, and xenograft growth while suppressing apoptosis. Mechanistically, it acts through miR-20a-5p sponging/inhibition, and circ_0009910 knockdown increases miR-20a-5p to suppress AML cell growth.

Confidence score:

0.8416

Other information
Title:

Silencing of circ_0009910 inhibits acute myeloid leukemia cell growth through increasing miR-20a-5p.

Journal: Blood cells, molecules & diseases
Published: 2019
PubMed ID: 30612066
Study type:

combined biological and clinical study

Data availability: -
Code availability: -