| Expression pattern: |
DN |
| Associated gene: |
- |
| Associated microRNA: |
- |
| Biological function: |
circ-SMAD7 inhibits ESCC cell proliferation and migration; knockdown promotes proliferation and migration, whereas overexpression inhibits these phenotypes. |
| Molecular mechanism: |
The precise molecular mechanism was not determined in this study. |
| Biological pathway or process: |
proliferation (inhibits); migration (inhibits) |
| Detected method: |
Q
M
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; Actinomycin D / DRB Stability Assay; RT-qPCR; Microarray; Clinical Sample Validation; Transfection; Colony Formation Assay; Wound Healing Assay; ROC Analysis |
| Clinical significance: |
circ-SMAD7 was associated with TNM stage and lymphatic metastasis, showed diagnostic potential with AUC 0.8588, and may serve as a biomarker for ESCC diagnosis, therapy, risk estimation, and prognosis. |
| Description: |
circ-SMAD7 is a human circRNA derived from intron 1 of SMAD7 and is down-regulated in ESCC plasma, tissues, and cell lines. Its low expression is associated with advanced TNM stage and lymphatic metastasis, while overexpression suppresses ESCC cell proliferation and migration, supporting its potential as a diagnostic and therapeutic biomarker. |
| Confidence score: |
0.7766 |