| Expression pattern: |
DN |
| Associated gene: |
NONO, c-Myc, GALNT2, GALNT6, MGAT1, hnRNPU |
| Associated microRNA: |
- |
| Biological function: |
Inhibits protein glycosylation, growth, invasion, and metastasis; tumor suppressor in gastric cancer. |
| Molecular mechanism: |
circ-hnRNPU binds NONO and induces its cytoplasmic retention/aggregation, thereby repressing nuclear NONO-mediated c-Myc transactivation and cytoplasmic NONO-facilitated mRNA stability of glycosyltransferases and hnRNPU. |
| Biological pathway or process: |
proliferation (inhibits); invasion (inhibits); metastasis (inhibits); ceRNA regulation (other) |
| Detected method: |
Q
B
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; Sanger Sequencing; RNase R Treatment; Northern Blot; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; RT-qPCR; RNA-seq; RNA Pull-Down; RIP (RNA Immunoprecipitation); Co-IP; Luciferase Reporter Assay; Actinomycin D / DRB Stability Assay; Transfection; MTT; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); H&E Staining; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
Low circ-hnRNPU levels were associated with poor survival outcome of gastric cancer patients. |
| Description: |
circ-hnRNPU (hsa_circ_0112825), derived from hnRNPU, is down-regulated in gastric cancer and acts as a tumor suppressor. It binds NONO and promotes NONO cytoplasmic retention, which suppresses NONO-dependent c-Myc transactivation and NONO-mediated mRNA stabilization of glycosyltransferases (e.g., GALNT2/GALNT6/MGAT1), thereby inhibiting glycosylation and malignant phenotypes (growth/invasion/metastasis). Low circ-hnRNPU expression is associated with poor patient survival. |
| Confidence score: |
0.8866 |