| Expression pattern: |
UP |
| Associated gene: |
HIF1A, G6PC3, RTN4IP1 promoter |
| Associated microRNA: |
miR-532-5p |
| Biological function: |
Hypoxia-induced circRTN4IP1 promotes HCC cell viability, proliferation, migration, invasion, and glycolysis; knockdown of circRTN4IP1 inhibits hypoxia-induced malignant progression and glycolysis. |
| Molecular mechanism: |
HIF1A binds the RTN4IP1 promoter and positively regulates circRTN4IP1 transcription; circRTN4IP1 acts as a miR-532-5p sponge to positively regulate G6PC3 expression. |
| Biological pathway or process: |
proliferation (promotes); migration (promotes); invasion (promotes); glycolysis (promotes); ceRNA regulation (other); HIF-1 signaling (other) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; Actinomycin D / DRB Stability Assay; RT-qPCR; Transfection; MTT; Transwell Assay; ChIP / ChIP-seq; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Western Blot; Bioinformatics Analysis |
| Clinical significance: |
May provide potential candidates for therapeutic targeting of hypoxia HCC progression. |
| Description: |
This study found that hypoxia up-regulates circRTN4IP1 in HCC cells. HIF1A transcriptionally induces circRTN4IP1, and circRTN4IP1 promotes hypoxia-associated HCC cell proliferation, migration, invasion, and glycolysis through the miR-532-5p/G6PC3 ceRNA axis. |
| Confidence score: |
0.7581 |