circRNA basic information
circBase ID: hsa_circ_0004015
Name: hsa_circ_CDK14
Synonym: circ_0004015
Host Gene: CDK14
Genomic location(hg19): chr7:90355880-90377090:+
Genomic location(hg38): chr7:90726566-90747775:+
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0005233
MONDO name: non-small cell lung carcinoma
Disease details: Non-small cell lung cancer / NSCLC
Disease DO ID:
 3908
Disease MeSH ID:
 D002289
Disease NCIt ID:
 C2926
Disease ICD11 ID:
 -
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

NSCLC tissues; corresponding normal tissues
Cell lines:

A549; H1975; H358; H1299; HCC827; 16HBE; HCC827I/R
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
UP
Associated gene: PDPK1
Associated microRNA: miR-1183
Biological function: Promotes NSCLC cell viability, proliferation, invasion, tumor growth, and gefitinib resistance.
Molecular mechanism: hsa_circ_0004015 functions as a miR-1183 sponge to relieve miR-1183-mediated repression of PDPK1, thereby promoting NSCLC progression and TKI resistance.
Biological pathway or process:

proliferation (promotes); invasion (promotes); drug resistance (promotes); ceRNA regulation (other)
Detected method:
Q
M
Validation methods:

Microarray; RT-qPCR; Clinical Sample Validation; Transfection; MTT; CCK8; Colony Formation Assay; Transwell Assay; In Vivo Animal Model; Luciferase Reporter Assay; RNA Pull-Down; IF (Immunofluorescence); Western Blot; Survival Analysis; Bioinformatics Analysis
Clinical significance:

High hsa_circ_0004015 expression is associated with poor overall survival and correlates with differentiation grade, invasion, and TNM stage in NSCLC patients.
Description:

hsa_circ_0004015 is up-regulated in NSCLC tissues and cell lines and is associated with poorer overall survival. Functionally, it promotes NSCLC cell viability, proliferation, invasion, xenograft growth, and gefitinib resistance. Mechanistically, it acts through the miR-1183/PDPK1 regulatory axis, consistent with a ceRNA-like oncogenic role.
Confidence score:

0.7761
Other information
Title:

Circular RNA hsa_circ_0004015 regulates the proliferation, invasion, and TKI drug resistance of non-small cell lung cancer by miR-1183/PDPK1 signaling pathway.

Journal: Biochemical and biophysical research communications
Published: 2019
PubMed ID: 30509491
Study type:

combined biological and clinical study
Data availability: Supplementary data; https://doi.org/10.1016/j.bbrc.2018.11.157; GEPIA online bioinformatics tool (http://gepia. cancer-pku.cn/detail.php?gene=&clicktag=boxplot)
Code availability: -