| Expression pattern: |
UP |
| Associated gene: |
CBX8 mRNA, CBX8 protein |
| Associated microRNA: |
miR-618 |
| Biological function: |
circ_0005230 functions as an oncogene in breast cancer cells, promoting cell growth, proliferation, migration and invasion, and suppressing apoptosis. |
| Molecular mechanism: |
circ_0005230 acts through a ceRNA mechanism by directly sponging miR-618, inhibiting miR-618 expression and thereby increasing CBX8 expression. |
| Biological pathway or process: |
ceRNA regulation (promotes); proliferation (promotes); apoptosis (inhibits); migration (promotes); invasion (promotes); metastasis (promotes) |
| Detected method: |
Q
|
| Validation methods: |
RT-qPCR; Clinical Sample Validation; Transfection; CCK8; Colony Formation Assay; Annexin V/PI Flow Cytometry; Transwell Assay; In Vivo Animal Model; IHC (Immunohistochemistry); Luciferase Reporter Assay; Western Blot; Cohort Study; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High circ_0005230 expression is associated with larger tumor size, advanced TNM stage, positive lymph node metastasis and worse overall survival, and circ_0005230 was identified as a prognostic predictor in breast cancer patients. |
| Description: |
circ_0005230 is up-regulated in breast cancer tissues and cell lines and is associated with aggressive clinicopathological features and poor overall survival. Functionally, it promotes breast cancer cell proliferation, tumor growth, migration and invasion while inhibiting apoptosis. Mechanistically, circ_0005230 sponges miR-618 to relieve repression of CBX8, forming a circ_0005230/miR-618/CBX8 oncogenic regulatory axis. |
| Confidence score: |
0.7528 |