circRNA basic information
circBase ID: hsa_circ_0000375
Name: hsa_circ_IFFO1
Synonym: CircIFFO1
Host Gene: IFFO1
Genomic location(hg19): chr12:6657590-6657991:-
Genomic location(hg38): chr12:6548424-6548825:-
Subcellular localization: not tested
 
 
 
 
 
 
 
Disease basic information
MONDO ID:
 0002529
MONDO name: skin squamous cell carcinoma
Disease details: Cutaneous squamous cell carcinoma / CSCC
Disease DO ID:
 3151
Disease MeSH ID:
 -
Disease NCIt ID:
 C4819
Disease ICD11 ID:
 1448983042
Disease OMIM ID:
 -
Species: Human
Species details: Homo sapiens
Tissue specimen:

CSCC tissues; para-cancer normal tissues; non-inflamed normal skin tissues; xenograft tumor tissues
Cell lines:

HaCaT; A431; SCC13
In vivo animal model:

cell line-derived xenograft
circRNA-disease information
Expression pattern:
DN
Associated gene: NFIB
Associated microRNA: miR-424-5p
Biological function: CircIFFO1 acts as a tumor suppressor in CSCC, inhibiting cell proliferation, migration, invasion, EMT and xenograft tumor growth, inducing G1/S cell cycle arrest and promoting apoptosis.
Molecular mechanism: CircIFFO1 functions as a molecular sponge for miR-424-5p and positively regulates NFIB expression, thereby suppressing malignant CSCC progression through the circIFFO1/miR-424-5p/NFIB axis.
Biological pathway or process:

proliferation (inhibits); migration (inhibits); invasion (inhibits); apoptosis (promotes); cell cycle (other); EMT (inhibits); metastasis (inhibits); ceRNA regulation (other)
Detected method:
Q
Validation methods:

RT-qPCR; Bioinformatics Analysis; Clinical Sample Validation; RNase R Treatment; Transfection; MTT; EdU Staining; Colony Formation Assay; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Transwell Assay; Western Blot; Luciferase Reporter Assay; RNA Pull-Down; RIP (RNA Immunoprecipitation); In Vivo Animal Model; H&E Staining; IHC (Immunohistochemistry)
Clinical significance:

CircIFFO1 might provide promising targets for CSCC treatment.
Description:

CircIFFO1 is down-regulated in CSCC tissues and cell lines and functions as a tumor suppressor. Mechanistically, it sponges miR-424-5p to up-regulate NFIB, thereby inhibiting proliferation, migration, invasion, EMT and xenograft tumor growth while promoting apoptosis. The circIFFO1/miR-424-5p/NFIB axis may represent a potential therapeutic target for CSCC.
Confidence score:

0.8171
Other information
Title:

CircIFFO1 suppresses tumor growth and metastasis of cutaneous squamous cell carcinoma by targeting the miR-424-5p/NFIB axis.

Journal: Archives of dermatological research
Published: 2023
PubMed ID: 37405427
Study type:

combined biological and clinical study
Data availability: GSE74758; Supplementary Table 1; Supplementary Fig. 1; Supplementary Fig. 2; Supplementary Fig. 3; Supplementary Fig. 4; https:// doi. org/ 10. 1007/ s00403-023-02659-6
Code availability: -