| Expression pattern: |
UP |
| Associated gene: |
LMO3, Ago2 |
| Associated microRNA: |
miR-337-3p |
| Biological function: |
promotes NSCLC cell proliferation and cell cycle progression, inhibits apoptosis, and promotes xenograft tumor growth in vivo |
| Molecular mechanism: |
circ_0007385 functions as a ceRNA/miRNA sponge for miR-337-3p, thereby up-regulating LMO3 expression in NSCLC cells |
| Biological pathway or process: |
ceRNA regulation (other); proliferation (promotes); cell cycle (promotes); apoptosis (inhibits) |
| Detected method: |
Q
|
| Validation methods: |
RNase R Treatment; RT-qPCR; Clinical Sample Validation; Nuclear-Cytoplasmic Fractionation; Transfection; CCK8; Colony Formation Assay; EdU Staining; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Western Blot; Luciferase Reporter Assay; RIP (RNA Immunoprecipitation); Bioinformatics Analysis; In Vivo Animal Model; IHC (Immunohistochemistry) |
| Clinical significance: |
high circ_0007385 expression was associated with advanced TNM stage, large tumor size, positive lymph node metastasis, and poorly differentiated histology grade, suggesting a potential prognostic biomarker for NSCLC patients |
| Description: |
circ_0007385 is up-regulated in NSCLC tissues and cell lines and acts as an oncogenic circRNA. It promotes proliferation and cell cycle progression, inhibits apoptosis, and supports xenograft tumor growth by sponging miR-337-3p to increase LMO3 expression. High circ_0007385 expression is associated with adverse clinicopathological features in NSCLC patients. |
| Confidence score: |
0.8175 |