| Expression pattern: |
UP |
| Associated gene: |
ROCK1 |
| Associated microRNA: |
miR584-3p |
| Biological function: |
Promotes SCLC cell migration and tumor metastasis, with little effect on proliferation, cell cycle, apoptosis or colony formation. |
| Molecular mechanism: |
FECR2 acts through a miR584-3p/ROCK1 pathway, consistent with cytoplasmic sequestration of miR584-3p and regulation of ROCK1 activity. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Bioinformatics Analysis |
| Clinical significance: |
FECR2 is highly expressed in SCLC tissues and its intensity is higher in patients with positive lymph node metastasis, indicating correlation with metastatic characteristics. |
| Description: |
FECR2 is a human FLI1-derived exonic circRNA up-regulated in SCLC tissues and enriched in the cytoplasm. It promotes migration and metastasis of SCLC cells through the miR584-3p/ROCK1 pathway and is associated with lymph node metastasis in SCLC patients. |
| Confidence score: |
0.8072 |