| Expression pattern: |
UP |
| Associated gene: |
ROCK1 |
| Associated microRNA: |
miR584-3p |
| Biological function: |
Promotes SCLC cell migration, invasion and tumor metastasis; serum exo-FECR1 tracks disease progression and chemotherapy response. |
| Molecular mechanism: |
FECR1 acts through a miR584-3p/ROCK1 pathway, consistent with a cytoplasmic miRNA-sequestering mechanism that promotes metastasis. |
| Biological pathway or process: |
migration (promotes); invasion (promotes); metastasis (promotes); ceRNA regulation (promotes); other pathway/process (promotes) |
| Detected method: |
Q
H
|
| Validation methods: |
Back-Splice Junction PCR / divergent primers PCR; RNase R Treatment; Sanger Sequencing; RT-qPCR; FISH / smFISH; Nuclear-Cytoplasmic Fractionation; Clinical Sample Validation; Luciferase Reporter Assay; Transfection; MTT; Cell Cycle Assay; Annexin V/PI Flow Cytometry; Colony Formation Assay; Transwell Assay; Western Blot; In Vivo Animal Model; H&E Staining; Survival Analysis; Bioinformatics Analysis |
| Clinical significance: |
High serum exo-FECR1 is associated with SCLC, extensive stage, shorter disease remission and dynamic chemotherapy response; serum exo-FECR1 may serve as a biomarker to track disease progression. |
| Description: |
FECR1 is a human FLI1-derived exonic circRNA up-regulated in SCLC tissues and serum exosomes. It promotes SCLC migration/metastasis through the miR584-3p/ROCK1 pathway, and serum exo-FECR1 is associated with disease stage, remission duration and chemotherapy response. |
| Confidence score: |
0.84 |